Monolithic flow reactor for enzymatic oxidations

BACKGROUND Oxidation is among the most important reactions in organic chemistry. Enzymatic oxidation offers a greener alternative to conventional chemo-catalytic approach and opens potential for new reactions. However, inefficient use of expensive enzymes oxygen (O2) limitations represent particular challenges biocatalytic reactor design. This work reports tubular continuous flow enzymatic oxidations. RESULTS The comprises thiol-functional porous monolith (0.93 ± 0.16 m2 g?1) an O2-permeable wall (115 600 1500 mL m?2 day?1). retains enzyme inside leading efficient use. acts as membrane contactor providing transport O2 from atmospheric air immediate proximity reactor, without any pressure sparging required. performance was demonstrated using glucose by oxidase (EC 1.1.3.4) coupled with situ consumption hydrogen peroxide horseradish peroxidase 1.11.1.7). At constant rate 0.1 min?1, product concentration reached 0.10 mmol L?1 after 1.5 h continued be relatively high next ?10 h. Overall, remained active >40 only 15 ?g oxidase. Furthermore, could rejuvenated periodic injection fresh thus can operate continuously extended periods. CONCLUSION We have shown here delivery. Moreover, its flexible design, optimized accommodate range gas-dependent transformations. © 2021 Society Chemical Industry (SCI).